phire plant direct pcr master mix yeast

phire plant direct pcr master mix yeast

PCR Phusion[]

2011-2-1 · ,Phusion® Flash High-Fidelity PCR Master Mix,kb 15 ...,——Phire® Plant Direct PCR Kit(Phire® PCR)。

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Thermo Scientific Direct PCR

2018-6-12 · Thermo Scientific Phire Plant Direct PCR Master Mix Reliable results at a wide range of amplicon lengths Figure 2. Different length amplicons (0.5 kb, 1.5 kb, and 5.0 kb) were amplified from dried Laurus spp. leaves. 0.5 mm punches of leaves were placed directly into Phire Plant Direct PCR Master Mix. M: O''GeneRuler Express DNA Ladder

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Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Pol

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Thermo Scientific Direct PCR

2016-10-14 · Thermo Scientific Phire Plant Direct PCR Master Mix Reliable results at a wide range of amplicon lengths Figure 2. Different length amplicons (0.5 kb, 1.5 kb, and 5.0 kb) were amplified from dried Laurus spp. leaves. 0.5 mm punches of leaves were placed directly into Phire Plant Direct PCR Master Mix. M: O''GeneRuler Express DNA Ladder

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Product Information Thermo Scientific Phire Plant Direct ...

2015-7-29 · 2X Phire Plant Direct PCR Master Mix has been optimized for Direct PCR from plant material. It contains the dNTPs and provides 1.5 mM MgCl 2 concentration in the final reaction. It also includes a density reagent and two tracking dyes for direct loading of PCR product on a gel. The Master Mix employes Phire Hot Start II DNA Polymerase, that ...

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Phire Plant Direct PCR MM :100x50μlrxns :F-160S : fermentas Phusion High-Fidelity PCR Master Mix with HF Buffer :500x50μl :F-531L : ...

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Thermo Scientific™ Phire Plant Direct PCR Master Mix 500 …

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, detailed protocols making it an ideal choice for amplification of plant DNA. When combined …

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Direct PCR from Yeast Cells

Phire Plant Direct PCR Master Mix - Thermo Fisher Scientific

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Colony Pcr Amplifying Genomic Dna Protocol

2021-7-27 · DNA synthesis. The results show Our results show that the Phire Plant Direct PCR Master Mix allows direct DNA amplification from yeast cells. Extraction of nucleic acids from yeast cells and plant tissues using ethanol as medium for sample preservation and cell …

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Phire Plant Direct PCR Master Mix

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria and yeast. The kit includes a …

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Thermo Scientific™ Phire Pflanzen-Direkt-PCR …

Der Phire Plant Direct PCR Master Mix wurde mit Blättern und Samen einer Vielzahl von Pflanzenarten getestet. Das Kit enthält einen kompletten Satz optimierter Reagenzien, detaillierte Protokolle und ist somit die ideale Wahl für die Amplifikation von Pflanzen …

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Direct to PCR. Fewer steps to discovery.

2018-8-30 · Phire Plant Direct PCR Master Mix* 100 x 50 μL rxns 500 x 50 μL rxns 250 x 20 μL rxns 1,250 x 20 μL rxns F-160S F-160L Phusion Blood Direct PCR Master Mix* 100 x 20 μL rxns 500 x 20 μL rxns NA NA F-175S F-175L * Also available as stand-alone kits. M 1 2 34 56 7 9810 11– + M

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Thermo Scientific Phire Plant Direct PCR Master Mix 250 x ...

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, detailed protocols making it an ideal choice for amplification of plant DNA. When combined …

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Direct PCR—Phire and Phusion Kits & Master Mixes | …

Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. The master mix contains Phire Hot Start II DNA Polymerase and is specially formulated for performing PCR from samples with plant-derived inhibitors such as complex polysaccharides and polyphenols.

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Thermo Scientific molecular biology workflow solutions

2021-4-14 · Direct PCR Master Mix Sample type Description Size . No. Direct protocol Dilution and storage protocol Animal and human tissues Phire Tissue Direct PCR Master Mix 100 rxns/500 rxns 250 rxns/1,250 rxns F170S/F170L Plant tissues, bacteria, yeast Phire Plant Direct PCR Master Mix 100 rxns/500 rxns 250 rxns/1,250 rxns F160S/F160L

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Direct PCR from Yeast Cells

2021-11-22 · The Phire Plant Direct PCR Master Mix allows analysis of yeast clones grown in liquid growth medium or on agar. A small aliquot of yeast liquid culture or a punch of a yeast colony is placed directly into master mix for fast and robust amplification. The master mix includes a density reagent

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Finnzymes_

2017-8-1 · Finnzymes F-122S Phire Hot Start II DNA Polymerase 200 x 50ul. Finnzymes F-130 Phire Plant Direct PCR Kit 200 x 50ul. Finnzymes F-140 Phire Animal Tissue Direct PCR 200 x 50ul. Finnzymes F-150 Phusion Human Specimen Direct 200 x 50ul. Finnzymes F-185L Harris Uni-Core/25-0.50mm Blue Bag of 25.

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Improved CRISPR/Cas9 gene editing by fluorescence ...

2019-6-17 · PCR-amplicons containing the NbRRA & NbPDS targets were amplified using nested PCR: First 5 μl of protoplast suspension (obtained as described in ''Protoplast isolation'') was used in a 50 μl PCR reaction using Phire Plant Direct PCR Master Mix (ThermoFisher F160S) with the cycle parameters: 5 min at 98 °C followed by 40 cycles of 10 s at ...

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Products > Laboratory Consumables > Molecular > Master …

Pre order. Thermo Scientific Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial. RM 845.49. Pre order. Thermo Scientific Phire Plant Direct PCR Master Mix. SPECIAL ONLINE PRICE! No: F160S. Phire Plant Direct PCR Master Mix. Unit Size: 250 rxns.

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2020-5-8 · (),CRONGS,、、、、,1.2。,:、、、3000 …

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Materials Tested Phire Plant Direct

2018-6-29 · The Thermo Scientific ™ Phire Plant Direct PCR Master Mix and Kit are designed to amplify DNA directly from plant samples. The following are examples …

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Thermo Scientific Phire Plant Direct PCR Kit

2015-7-29 · 5.2. Phire Plant PCR Buffer The 2X Phire Plant PCR Buffer has been optimized for direct PCR from plant material. It contains the dNTPs and provides 1.5 mM MgCl2 concentration in the final reaction. 5.3. Dilution Buffer The Dilution Buffer has been optimized to release DNA from a wide variety of different sample materials such as plant leaves ...

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Takara Bio PCR products

Your PCR polymerase needs to amplify reliably regardless of your target and sample type. Our scientists have developed a wide range of the highest-performing PCR enzymes and blends with optimized formulations to meet routine and challenging reaction conditions—from long and accurate PCR to fast PCR to inhibitor-resistant direct PCR.

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Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. …

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Thermo ScientificPhire Plant Direct PCR Master Mix:PCR ...

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, detailed protocols making it an ideal choice for amplification of plant DNA. When combined …

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Direct PCR from Yeast Cells

2018-9-5 · Direct PCR from yeast was done using Phire Plant Direct PCR Master Mix (F-160S). For direct PCR on yeast grown in liquid culture, 1 µL of overnight culture was added to 50 µL of 1X Phire Plant Direct PCR Master Mix. For direct PCR on yeast grown agar plates, yeast cells were grown on agar plates overnight to allow formation of colonies. A ...

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Genetic structure of Fusarium verticillioides populations ...

2021-11-1 · The final volume of 20 µL contained 1X Phire Plant Direct PCR Master Mix (composed by Phire Hot Start II DNA Polymerase, dNTPs and MgCl2), 0.5 µM of each primer and 0.5 µL of genomic DNA isolated as previously described. The PCR conditions were optimized as follows: a first denaturation at 98 °C for 5 min, 30 cycles of 5 sec at 98 °C, 5 ...

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T7 R&DNA™ Polymerase, T7 R&DNA™

Phire Plant Direct PCR Master Mix : PCR DNA : 1,250 reactions,250 reactions F160L,F160S RevertAid First Strand cDNA Synthesis Kit/cDNA cDNA; 42°C; ...

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molecular biology

2018-12-28 · Phire Plant Direct PCR Master Mix 250 x 20 µL rxns ¥1,412 ¥918 F160L 1250 x 20 µL rxns ¥6,231 ¥4,050 F175S Phusion Blood Direct PCR Master Mix 100 x 20 µL rxns ¥1,097 ¥713 F175L 500 x 20 µL rxns ¥4,322 ¥2,809 dNTP 2 mM R0241 dNTP Mix (2 ...

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The Phylogeny of Camellia sinensis Accessions

2021-1-6 · 3. Prepare PCR solution using 2x phire plant direct PCR master mix and Bel-1 and Bel-3 primers. 4. Run PCR using conditions specified in Lee et al. paper. 5. Run a gel electrophoresis using 10 µl of PCR solutions and 100 bp ladder. 6. Elute DNA from gel and send to sequencing lab. 7. Compare sequences using MEGA X software and

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PCR | Thermo Fisher Scientific

Phusion Blood Direct PCR Master Mix. 500 x 20 µL rxns. ¥3,898. . . Thermo Scientific™ PCR.,PCR. . PCR,.

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Thermo Scientific™ Phire Plant Direct PCR Master Mix 250 …

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, detailed protocols making it an ideal choice for amplification of plant DNA. When combined …

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